Figure 1: miR863-3p is induced by Pst carrying the effector avrRpt2. | Nature Communications

Figure 1: miR863-3p is induced by Pst carrying the effector avrRpt2.

From: miRNA863-3p sequentially targets negative immune regulator ARLPKs and positive regulator SERRATE upon bacterial infection

Figure 1

(a) miR863-3p and (b) miR863-5p levels in mock-, Pst (avrRpt2)-, Pst (ΔhrcC)- and Pst (EV)-infected Col-0 WT plants were detected by northern blot analysis. Bacterial inoculum concentration: 1 × 107 c.f.u. per ml. Leaf tissue was collected at 14 h.p.i. U6 was used as a loading control. Relative abundance (RA) levels are indicated. Similar results were obtained from three biological replicates. (c) miR863-3p levels in various Pst (avrRpt2)-infected mutants defective in miRNA biogenesis (Ler dcl1-7/fwf2 and Col-0 se-1) or siRNA biogenesis (Col-0 rdr6-15) are detected by northern blot analysis. Mock-treated and Pst (avrRpt2)-infected Col-0 WT and Pst (avrRpt2)-infected Ler WT were used as controls. Bacterial inoculum concentration: 1 × 107 c.f.u. per ml. Leaf tissue was collected at 14 h.p.i. U6 was used as a loading control. RA levels are indicated. Similar results were obtained from three biological replicates.

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