Figure 5: ARLPKs and AKIK1 predominantly localize in the ER.

(a) Co-localization of ARLPK1-YFP or ARLPK2-YFP with ER-mCherry in N. benthamiana was observed with confocal microscopy. Untreated plants were visualized 60 h after co-infiltration. Treated plants were infected 48 h after co-infiltration with Pst (avrRpt2) and visualized 12 h.p.i. Bacterial inoculum concentration: 1 × 10⁷ c.f.u. per ml. Fluorescent images of the middle of the cell were taken separately then merged. Samples were visualized without (−NaCl) and with (+NaCl) plasmolysis treatment (5% NaCl for 5–10 min). Scale bars, 20 μm. (b) Co-localization of AKIK1-CFP with ER-mCherry and ARLPK1-YFP with AKIK1-CFP in N. benthamiana was observed with confocal microscopy. Untreated plants were visualized 60 h after co-infiltration. Treated plants were infected 48 h after co-infiltration with Pst (avrRpt2) and visualized 12 h.p.i. Tissue was collected for imaging 12 h.p.i. Bacterial inoculum concentration: 1 × 10⁷ c.f.u. per ml. Fluorescent images of the middle of the cell were taken separately then merged. Samples were visualized without (– NaCl) and with (+NaCl) plasmolysis treatment (5% NaCl for 5–10 min). Scale bars, 20 μm.