Figure 7: Chemical shift differences following a titration of 15N-labelled RXFP1(1–72) with 20 molar equivalents of EL1(475–486)/EL2-RXFP1. | Nature Communications

Figure 7: Chemical shift differences following a titration of 15N-labelled RXFP1(1–72) with 20 molar equivalents of EL1(475–486)/EL2-RXFP1.

From: The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1

Figure 7

(a) portion of 1H-15N HSQC spectrum of RXFP1(1–72) showing chemical shift effects on titration with EL1(475–486)/EL2-RXFP1 (b) chemical shift differences for wild-type RXFP(1–72) (black), F54A (blue) and G41A/D42A (red) (c) wild-type RXFP(1–72) (black), G45A/W46A (blue) and C40Ains (red). Experiments were conducted at pH 6.8 and 25 °C.

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