Figure 9: Decreased glucose uptake and Chrebpβ-driven DNL is a primary consequence of Rictor loss in adipocytes.
From: Adipose tissue mTORC2 regulates ChREBP-driven de novo lipogenesis and hepatic glucose metabolism
(a) Western blots of indicated proteins at different days of differentiation using Rictor iKO primary adipocytes (described in Methods). (b) Oil Red O staining of differentiated adipocytes. (c) The relative mRNA level of ChREBPβ in differentiated cells at various time points. n=3. (d) 2-DG uptake in differentiated adipocytes without or with insulin stimulation. n=3. (e) The C14-glucose-derived FA in differentiated adipocytes. n=3. (f) The C14-glucose derived triglyceride (TAG) in differentiated adipocytes. n=3. (g) Relative glut4 expression in differentiated cells at various time points. n=3. (h) Relative glut4 expression in sWAT. n=8. (i) Glycerol release in differentiated adipocytes under basal and isoproterenol (Iso) stimulation. n=4. (j) Glycerol release in ex vivo pgWAT under basal and isoproterenol stimulation. n=6. Data were analysed by Student’s t-test. Values are expressed as mean+s.e.m. *P<0.05; **P<0.01; ***P<0.001.
