Figure 2: PDGF-BB-PDGFRβ-signalling induces IL-33 expression.

(a) Heatmap of top 10 most upregulated and downregulated genes by genome-wide expression profiling of PDGF-BB-stimulated lung pericytes cultured in vitro. (b) Heatmap of top 10 most upregulated and downregulated inflammation-related signalling molecules by genome-wide expression profiling of PDGF-BB-stimulated lung pericytes cultured in vitro. (c) qPCR quantification of Il33 mRNA expression levels in PDGF-AA- or PDGF-BB-stimulated lung pericytes cultured in vitro. (PC; n=6 samples per group). NS, not significant. (d) qPCR quantification of Il33 mRNA expression levels in PDGF-BB-stimulated bone marrow stromal fibroblasts cultured in vitro (SF; n=6 samples per group). (e) Quantification of mouse IL-33 protein levels of vector- and PDGF-BB-T241 tumours, and scrambled and Pdgfb shRNA-transfected A431 tumours (n=6 samples per group). (f) qPCR quantification of Il33 mRNA of Adv-Gfp- and Adv-Pdgfb-infected liver tissues (n=6 samples per group). Adv-Gfp-infected hepatocytes were visualized by a fluorescent microscope. (g) qPCR quantification of Il33 mRNA expression levels in CD31⁺, PDGFRβ⁺ and NG2⁺ cell populations isolated from vector and PDGF-BB T241 tumours (n=6 samples per group). Vector and PDGF-BB T241 tumour cells served as controls. (h) qPCR analysis of Il33 mRNA of vehicle-, anti-PDGFRα-, anti-PDGFRβ- or imatinib-treated PDGF-BB-stimulated or non-stimulated lung pericytes cultured in vitro. (PC; n=6 samples per group; mean±s.e.m., NS, not significant, Student’s t-test).