Figure 6: Aurora A inhibition impairs TCR signalling pathways.
From: Aurora A drives early signalling and vesicle dynamics during T-cell activation
(a) Immunoblottings showing phosphorylation of the indicated molecules in lysates of J77 Jurkat T cells pretreated with vehicle (DMSO) or Aurora A inhibitor (MLN8237) and conjugated for the indicated times with SEE-pulsed Raji B cells. (b) Quantification of blots as in a from four to six independent experiments. Error bars represent interquartile range. Medians were compared with a Friedman test (*P<0.05). n.s., nonsignificant. (c) Immunoblottings showing phosphorylation of the indicated molecules in lysates of DMSO- or MLN8237-pretreated primary human CD4+ T cells conjugated for the indicated times with anti-CD3/CD28-coated beads. (d) Quantification of blottings as in c from four to six independent experiments. Error bars represent interquartile range. Medians were compared with a Friedman test (*P<0.05). n.s., nonsignificant.
