Figure 4: Transcription profile of Mtb residing in biofilms.
From: Thiol reductive stress induces cellulose-anchored biofilm formation in Mycobacterium tuberculosis
RNA was isolated from planktonic Mtb cultures at an OD600 of 0.8 and from the mature Mtb biofilms resulting from the response to TRS. The expression profiles were determined using high-density oligonucleotide array analysis. Heat maps of significantly differentially expressed genes (SDEGs) with average fold changes of ⩾1.5 were created. (a) Heat map of the genes upregulated in Mtb biofilms compared with planktonic growth. (b) Heat map of the genes downregulated in response to DTT-induced reductive stress. The intensity of the green and red colours indicates the fold changes in gene expression. (c) SDEGs with average fold change of more than 1.5-fold on biofilm formation were classified into ten classes based on their annotations in TubercuList. Pie chart depicts the relative share (in percentage) of various pathways modulated in Mtb biofilm compared with planktonic bacteria.
