Figure 1: Optical recordings from MCH and OH cells in awake freely moving mice
From: Awake dynamics and brain-wide direct inputs of hypothalamic MCH and orexin networks
(a) Scheme for targeting GCaMP6s expression. (b,c) Coronal brain slices from Pmch-Cre and orexin-Cre mice showing fibre locations (yellow dashed lines) and injection sites. DMH, dorsomedial hypothalamus; LH, lateral hypothalamus; 3V, third ventricle; VMH, ventromedial hypothalamus. Representative images of n=6 brains per group. Scale bars, 500 μm. (d) Left, scheme for fibre photometry. Right, calcium signals during cage exploration from mice expressing GCaMP6s in MCH neurons or GCaMP6s or eGFP in OH neurons (representative examples of n=4, 4, and 3 mice, respectively). (e) Calcium signals from mice expressing GCaMP6s in MCH and OH cells, aligned to application of tail air-puff (a.p.). Turquoise or magenta lines indicate means, grey lines indicate s.e.m., n=4 mice per group. (f) Quantification of data in e. Changes in fluorescence (means±s.e.m., relative to values at −60 s) that occurred before a.p. (−20 to 0 s) and after the a.p. (0 to 20 s). Numbers above bars are P values from paired t-tests (MCH network: t, d.f.=0.367, 3; OH network t, d.f.=13.169, 3; n=4 mice per group).
