Figure 2: Differential modulation of MCH and OH cells by sensory experiences.
From: Awake dynamics and brain-wide direct inputs of hypothalamic MCH and orexin networks
(a) Calcium signals from mice expressing GCaMP6s in MCH or OH cells aligned to novel object (n.o.) presentation (top and middle plots) or to the time of object area entry (a.e.; bottom plot). Turquoise or magenta lines are means, grey lines are s.e.m., n=4 mice per group. (b) Binned quantification of data in a (top plots) and object area entry (bottom plot). Changes in fluorescence (means±s.e.m., black) and individual values, relative to values at −2 min, before and after n.o. appearance (at indicated times). Numbers above data are P values of Dunnett’s multiple comparisons tests following one-way repeated measures analyses of variance (ANOVAs; MCH network: F(6,18)=16.67, P<0.0001; OH network: F(6,18)=31.79, P<0.0001). n=4 mice per group. (c) Calcium signals from mice expressing GCaMP6s in MCH or OH cells aligned to immobilization stress (i.s.) applied for 1 min. Turquoise or magenta lines are means, grey lines are s.e.m., n=4 mice per group. (d) Quantification of data in c. Changes in fluorescence (means±s.e.m., relative to values at −2 min, before, during and after i.s. (at indicated times). Numbers above data are P values of Dunnett’s multiple comparisons tests following one-way repeated measures ANOVAs (MCH network: F(2,6)=5.7, P=0.041; OH network: F(2, 6)=27.77, P=0.0009). n=4 mice per group.
