Figure 5: Pharmacological inhibition of AURKA or PLK-1 prevents the formation of binucleated cells.
From: Essential role for a novel population of binucleated mammary epithelial cells in lactation
(a) Bar graph showing percentage of cells containing 2N or 4N DNA content in the luminal, basal and stromal cell compartments from mice treated from 16.5 dP with vehicle, MLN8237 (AURKA inhibitor) or BI6727 (PLK-1 inhibitor) and analysed by FACS at 2 dL. Error bars represent mean±s.e.m. (n=3 mice per treatment). ***P<0.001. (b) Representative FACS plots of DNA ploidy in the luminal populations from a. (c) Representative whole-mount 3D confocal images of ductal portions and optical sections from the enlarged regions for mice treated with vehicle or MLN8237. (d) Representative whole-mount 3D confocal images of ductal portions and optical sections from the enlarged regions for mice treated with vehicle or BI6727. In c and d, the whole-mounts were labelled for DAPI (white) and F-actin (red). In the right panels, nuclei in mononucleated cells within the middle panels are depicted schematically as orange dots. Scale bars: 100 μm (whole-mounts); and 20 μm (optical sections).
