Figure 4: miR-424(322) influences IFN-γ induced PD-L1-associated CD8+ T-cell apoptosis in a Skov3 (CP)/T-cell co-culture model. | Nature Communications

Figure 4: miR-424(322) influences IFN-γ induced PD-L1-associated CD8+ T-cell apoptosis in a Skov3 (CP)/T-cell co-culture model.

From: miR-424(322) reverses chemoresistance via T-cell immune response activation by blocking the PD-L1 immune checkpoint

Figure 4

Skov3 (CP) cells with stable overexpression of miR-424(322) or miR-Src were first exposed to IFN-γ for 24 h in the presence or absence of anti-PD-L1. (a) Culture media were assayed for IL-10 using a cytokine ELISA assay. t-test, **P≤0.01. The results represent the mean±s.e.m. from three independent experiments. (b,c) T cells were subsequently co-cultured with mitomycin C-treated Skov3 (CP) cells for 24 h. T cells were sorted by FACS, stained with PE anti-PD-L1, Alexa Fluor 488 anti-annexin V and APC anti-CD8, and analysed by flow cytometry for T-cell apoptosis in the PD-L1+/CD8+ population. t-test, **P≤0.01. The results represent the mean±s.e.m. from three independent experiments, and the densitometric level of the apoptosis ratio is shown.

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