Figure 5: C-di-GMP directly binds CckA in a PAS domain-dependent manner.
From: A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues
(a) SPR experiments show that CckA directly binds c-di-GMP. Purified CckA ΔRD was tethered to the SPR chip and exposed to increasing concentrations of injected c-di-GMP. The binding period is displayed as response units plotted as function of time (t=2–62 s), followed by buffer injection to remove c-di-GMP. Increasing response curves were obtained using the increasing c-di-GMP concentrations shown on the right. (b–f) Additional SPR experiments examining the interaction of c-di-GMP with (b) CckA ΔCA ΔRD, (c) ΔDHp ΔCA ΔRD, (d) ΔPAS-A ΔRD, (e) ΔPAS-B ΔRD and (f) ΔPAS-A ΔPAS-B ΔRD.
