Figure 1: Generation of NMR-iPSCs from adult fibroblasts.

(a) Adult NMR. (b) Morphology of NMR-fibroblasts. (c) NMR-iPSCs (clone 27). (d) AP activity. (e) Karyotype of NMR-iPSCs (clone 24) at passage 10. (f) RNA-seq of expression levels of selected pluripotency and fibroblast markers in NMR-iPSCs and NMR-fibroblasts. Y axis: ratio of the average value of fragments per kilobase of transcript per million mapped reads (FPKM) of four NMR-iPSC clones to the average of NMR-fibroblast lines. (g) Immunocytochemical analyses of the expression of differentiated EBs is shown as follows: mesoderm (DESMIN, α-smooth muscle actin (αSMA)), endoderm (FOXA2 and VIMENTIN) and ectoderm (NESTIN and GFAP) markers. (h) Tumours or testes after transplantation of human-iPSCs (10 weeks), Ms-iPSCs (4 weeks) or NMR-iPSCs (10 or 20 weeks) into the testes of NOD/SCID mice. (i) Weights of tumours and testes. Ten weeks (n=20), 20 weeks (n=16) or 28 weeks (n=10) for NMR; 10 weeks (n=8) for human, 4 weeks (n=8) for mouse. n: transplanted testes. Y axis: weights in 6+log₂ arbitrary units. The data are represented as mean±s.e.m. *P<0.05; NS, not significant; Kruskal–Wallis test followed by the Dunn’s test. Scale bar, 200 μm.