Figure 8: The E3/49K proteins modulate targeted receptor function. | Nature Communications

Figure 8: The E3/49K proteins modulate targeted receptor function.

From: The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation

Figure 8

(a) Binding of HAdV-D43 and -D53 E3/49K proteins (100 nM) to wt (right) EPHA3-transfected (left) cells visualized by immunofluorescence. (b) Actin cytoskeleton and tyrosine phosphorylation changes upon stimulation with ephrin-A5, the viral proteins indicated or the combination of both. Assay shown is representative of two independent experiments. (c) Effects on adhesion of HEK293T cells to FN-coated plates. The cells were mock-treated or pre-incubated with the indicated viral proteins (100 nM), stimulated with Ephrin-A5 and incubated on FN-coated plates. Plots represents one assay run in quadruplicates out of two independent experiments. HAdV-D43 E3/49K protein counteracts ephrin-A5 effect on cell adhesion (Students t-test, (P<0.0001). Mean±s.d. is represented in all cases. Scale bars, 20 μm.

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