Table 1 Overview of physiological parameters for both strains under both growth conditions.

From: Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains

 

Batch

Chemostat

 

CEN.PK 113-7D

YSBN2

CEN.PK 113-7D

YSBN2

Specific growth rate (h−1)

0.40±0.01

0.301±0.002

0.099±0.001

0.097±0.001

Biomass concentration (g −1)

1.9±0.1

1.9±0.2

3.92±0.01

4.35±0.04

Extracellular glucose concentration (g l−1)

17.4±0.6

15±1

0.033±0.002

0.032±0.008

qGluc (mmol gDW−1 h−1)

−16±1

−13.0±0.5

−1.043±0.009

−0.93±0.01

qEth (mmol gDW−1 h−1)

23±3

19±3

−0.165±0.001

−0.146±0.002

qGlyc (mmol gDW−1 h−1)

0.89±0.02

1.19±0.07

NA

NA

qO2 (mmol gDW−1 h−1)

−2.6±0.3

−1.06±0.02

−2.74±0.03

−2.11±0.04

qCO2 (mmol gDW−1 h−1)

26.6±0.1

23±1

2.71±0.02

2.09±0.04

RQ (−)

10.1±0.9

21±1

0.990±0.004

0.99±0.01

Ys/x (Cmol Cmol−1)

0.16±0.01

0.146±0.005

0.568±0.002

0.630±0.006

qH+ (mmol gDW−1 h−1)

−3.1±0.3

−2.3±0.1

0.65±0.01

0.60±0.02

Carbon balance recovery

94±11%

98±9%

97.9±0.1%

98.7±0.5%

Degree of reduction balance recovery

94±15%

96±11%

95.7±0.5%

97.5±0.9%

  1. Data are averages and s.d.s between triplicate cultures. The yield coefficient for biomass is calculated based on a molecular weight for biomass of 26.4 g Cmol−1 (ref. 30). NA, glycerol was not detectably produced under chemostat conditions.