Figure 2: Inner ear organoids generated from Atoh1/nGFP ES cells include nGFP+ cells that expressed multiple hair cell markers. | Nature Communications

Figure 2: Inner ear organoids generated from Atoh1/nGFP ES cells include nGFP+ cells that expressed multiple hair cell markers.

From: Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells

Figure 2

(a,b) The appearance of nGFP+ cells in the epithelium of organoid vesicles on day 12 of differentiation. (c) At later stages of differentiation (>day 14), numerous nGFP+ cells populated the epithelium, typically in a regional cluster as seen in d and e. (f,g) Most nGFP+ cells were also Anx4a+ and Calretinin (Calb2)+. Arrowheads in g denote nGFP+ cells devoid of Calb2. (h,i) nGFP+ hair cells had acetylated-tublin+ kinocilia and F-actin+ hair bundles. (j,k) Most Calb2+ hair cells also had Espin+ hair bundles. (l) nGFP+ cells were also Myo7a+ and Sox2+. Scale bars, 100 μm (a,c,d and e), 25 μm (b) and 10 μm (f–l).

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