Figure 5: Atoh1/nGFP organoid hair cells have features that typify vestibular hair cell synapses. | Nature Communications

Figure 5: Atoh1/nGFP organoid hair cells have features that typify vestibular hair cell synapses.

From: Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells

Figure 5

(a,b) Confocal image of nGFP+ organoid hair cells with CtBP2+ puncta that colocalize with TUJ1+ neuronal processes. Scale bars, 50 μm (a), 20 μm (b). (c) Quantitative colocalization analysis of segmented CtBP2+ punta and nGFP+ nuclei. (d) A non-inactivating Ca2+ inward current evoked by the voltage step shown below. (e) Ca2+ current–voltage relations from three organoid hair cells. (f) Ca2+ currents evoked by a step to −23 mV were blocked by 200 μM Cd2+. (g) A family of Ca2+ currents evoked by the voltage protocol shown below. Subtraction of traces recorded in 200 μM Cd2+ from control traces revealed the Ca2+ currents shown. Activation kinetics were fit with a single exponential with a time constant of 1.1 ms at −25 mV (red line).

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