Figure 2: Development of fluorescent reporters for single-cell measurements.
From: Nucleation and spreading of a heterochromatic domain in fission yeast
A nuclear-targeted YFP under control of the ura4+ promoter was expressed from two sites in the mating-type region, respectively, (EcoRV) near the centromere-distal edge of the heterochromatic region and kint2 within the cenH element. The location of these sites is depicted in Figs 3 and 4. mCherry was expressed in the same cells from the ura4+ euchromatic locus. (a) Fluorescence of kint2::YFP, (EcoRV)::YFP and ura4::mCherry in clr4Δ and clr4+ cells. Scale bar, 5 μm. (b) Distribution of fluorescence intensities for the same strains, obtained by measuring nuclear fluorescence in microscopy images.
