Figure 1: ADAM17 stimuli induce PS exposure.
From: Phosphatidylserine exposure is required for ADAM17 sheddase function
(a,b) HaCaT keratinocytes were stimulated with melittin (Mel, 1 μM) or IO (1 μM) and analysed for PS exposure via live-cell imaging using the polarity-sensitive PS-binding dye pSIVA. The mean fluorescence area was quantified for statistical analysis (Mel: n=5; ±s.e.m.; IO: n=3; ±s.e.m.). (c,d) HaCaTs were stimulated with PMA (300 ng ml−1) or FGF7 (100 ng ml−1) and stained with Annexin V–FITC after the indicated time points. The mean fluorescence area was quantified for statistical analysis (n⩾3; ±s.e.m.). Unstimulated cells were used as control (squares). Scale bars, 10 μm.
