Figure 1: Multigene expression in primary cells by MultiPrime.
From: Highly efficient baculovirus-mediated multigene delivery in primary cells
HUVEC, REF cells and rat cortical neurons were infected with a MultiPrime baculovirus encoding EBFP2-Nuc (labelling the nucleus), mTFP1-FYVE (PI-3-P containing endosomes), tubulin-EYFP (cytoskeleton), Mito-dsRed (mitochondria) and PLCδ-PH (PI-4,5-P2; plasma membrane). iPS cells were transduced with a virus encoding mTFP1-actin, EYFP-tubulin and Mito-dsRed. Oct4 was used as a marker for pluripotent stem cells. All infected cells express all heterologous proteins. Scale bar, 20 μm.
