Figure 3: Transduction efficacy.
From: Highly efficient baculovirus-mediated multigene delivery in primary cells
(a) Transduction by MultiPrime baculovirus was compared to plasmid-based transfection. MultiBacMam bacoluvirus expressing VSV-G and EMBacY baculovirus devoid of VSV-G were used. For plasmid-based transfection, the plasmid (13 kb) used originally inserted into the recombinant baculoviruses was utilized. MultiPrime-mediated transduction is markedly superior in all cell types tested. Data shows mean value±s.d.; n=3; ****P<0.0001 determined by comparing transfection with transduction with or without VSV-G using one way analysis of variance followed by the Dunnet’s post hoc test. (b) Effects of the MOI are shown. (c) PAE cells were infected with a MultiPrime baculovirus expressing three proteins at a MOI of 500. DAPI was used to counterstain nuclei of all cells. Virtually all cells are infected and express all heterologous proteins. Scale bar, 100 μm. (d) Toxicity of transduction was measured by means of a MTT assay. MultiPrime transduction exhibits comparable, low toxicity as plasmid transfection. (e) The persistence of heterologous expression was quantified by fluorescence in REF cells. The percentage of positive cells for each individual protein is shown over time.
