Table 1 Proteomic analysis of proteins copurified with CATSPER1.

From: A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa

Band number

Protein name

Accession no.

No. of peptides

Coverage (%)

Testis specific

9

CATSPER1

21314844

8

19.7

8

CATSPER2

124001554

4

7.9

3

CATSPER3

30350218

6

17.6

4*

CATSPER4

29244466

7

18.5

16

CATSPERβ

257900479

34

39.3

14

CATSPERγ

157951745

7

8.3

12

TMEM146

261245039

2

2.7

12

Unnamed protein product (hypothetical protein LOC75721)

34147101

2

3.1

11

HSPA2 (HSP70-2)

123621

15

32.9

Not testis-specific

13*

PARASPECKLE

225543409

8

14.1

12

SLC39A6

26324938

4

7.1

11*

GRP78

1304157

17

34.4

8

Non-POU-domain-containing, octamer-binding protein

12963531

13

12.6

7

Cleavage and polyadenylation specificity factor subunit 7

24416453

14

21.6

6*

Tubulin β-2c

22165384

4

10.4

5*

SLC39A7

6680147

3

15.9

5*

Elongation factor Tu

556301

4

8.4

2*

Cleavage and polyadenylation specificity factor subunit 5

13386106

15

90.3

  1. *Proteins identified from only one of two purifications.
  2. Proteins verified to be in the CATSPER1 complex are in bold. Proteins tested but not found to be in the CATSPER1 complex are listed in italics. All other proteins were not further characterized.