Figure 5: Formation of ETs after incubation of infected erythrocytes with macrophages.
From: The TatD-like DNase of Plasmodium is a virulence factor and a potential malaria vaccine candidate
Representative images of the extracellular network structures after incubation of J774A.1 macrophages with WT P. berghei (a) and ΔPbTatD (b) infected erythrocytes. The ETs and dead cell nuclei were stained with Sytox green. Scale bar, 20 μm. (c) The relative quantification of ETs released by J774A.1 macrophages stimulated with the WT strain (white bar), ΔPbTatD strain (blue bar), PbTatD-com strain (yellow bar), PbTatD-D352A strain (green bar) or RBCs (red bar) are shown. The WT group was regarded as 100%. The results are the averages of three independent experiments (mean ±s.e.m., *P<0.05 in two-tailed Student’s t-test). (d,e) Images of ETs after the addition of rPbTatD-GST or rPbTatD-D352A-GST in the cultivation at various time points. rPbTatD-GST showed DNase activity in a time-dependent manner (d), whereas rPbTatD-D352A-GST did not show any activity (e).
