Figure 2: LASV neutralization by mAbs and assignment of competition groups. | Nature Communications

Figure 2: LASV neutralization by mAbs and assignment of competition groups.

From: Most neutralizing human monoclonal antibodies target novel epitopes requiring both Lassa virus glycoprotein subunits

Figure 2

Neutralizing activity of mAbs was evaluated in two pseudovirus assays and by a PRNT. (a) LASVpp expressing GPC representing the four LASV lineages (I–IV) and the HIV-1 core. (b) LASVpp expressing GPC representing LASV lineage IV and the LCMV core. (c) PRNT. The inset indicates the potency (heat map) for IC50 and IC80 neutralization by each of the mAbs tested in a–c. All three pairwise correlation coefficients were significantly different at the 5% significance level according to both Pearson’s and Spearman’s approaches. (d) Cross-competition analysis was performed pairwise with biotinylated (Biot.) mAbs. The analysis places neutralizing mAbs into four competition groups. 8.9F competes with itself and its binding is blocked by 12.1F. Results in Fig. 2 are representative of at least two experiments performed for each assay.

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