Figure 3: Tailoring smooth acoustic impedance gradients.
From: Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping
(a and b) Confocal cross sectional y-z scans recorded 20 mm downstream from the inlet, of the fluorescent dextran tracer gradient (blue color plot) in the case of (a) an iodixanol gradient and no sound, and (b) an iodixanol gradient and sound (red lines), along with schematic interpretations of the data. (c) Top views of the fluorescent dextran tracer gradient imaged with epifluorescence microscopy at different volume flow rates Qtot. The graph shows the corresponding normalized fluorescence intensity profiles versus y averaged along the flow direction x. (d) Top view epifluorescence images when varying the relative flow rate in the central Qc and side inlets while maintaining a constant overall flow rate Qtot and the corresponding normalized fluorescence intensity profiles. Scale bars (magenta) are 100 μm.
