Figure 2: Effects of all possible single mutations on alternative splicing of Fas exon 6.
From: The complete local genotype–phenotype landscape for the alternative splicing of a human exon
High mutational plasticity of alternative splicing. (a) Dotchart of the enrichment scores for all 189 single mutation variants. Colour code indicates whether the variant’s effect is statistically different from that of WT sequence (red indicates increased inclusion, blue increased skipping). (b) Heatmap displaying the extent of statistical difference from wt sequence for all 189 variants. Colour scale represents the log10 of the P value multiplied by 1 or −1 for variants with negative and positive enrichment scores, respectively. (c) Distribution of enrichment scores for all single mutants. The vertical black line represents the median of the distribution. PSI values were estimated from the enrichment scores using the relationship shown in Supplementary Fig. 3B.
