Figure 7: The interaction between bacteria and FYT21.

(a) Thrombin was digested with P. aeruginosa elastase and the resulting material was used in a bacterial overlay assay to assess bactericidal activity against E. coli. FYT21 was run in parallel; the lane is from the same gel but aligned for clarity. (b) Antimicrobial activity of FYT21 or HVF18 against various Gram-negative and Gram-positive bacteria in exponential phase in 10 mM Tris buffer containing 5 mM glucose and/or supplemented with 0.15 M NaCl or 0.15 M NaCl and 10% citrate plasma (cp), or (c) in 25% human whole blood and incubated for 2 h with a range of FYT21 or HVF18. Results are means±s.e.m. of 4 experiments. (d) P. aeruginosa 15159 was incubated with plasma (P), acute wound fluid (AWF) or chronic wound fluid (CWF) for 30 min and binding of thrombin-derived C-terminal epitopes to the bacterial membrane was visualized using electron microscopy (scale bars, 2 μm (overview image) and 100 nm).