Figure 2: Prospective isolation and characterization of human prostate TICs.
From: Tumour-initiating stem-like cells in human prostate cancer exhibit increased NF-κB signalling
(a) Percent marker expression in parent tumours (red) or the sequentially derived primary (green) and secondary (blue) spheres. Mean±s.d. (n=10). (b) Primary sphere formation is affected by the markers expressed in tumour cells (primary spheres/5,000 tumour cells). Mean±s.d. (n=10). (c, d) Percent coexpression of TRA-1-60 and (c) human-specific-PSMA or -EpCAM or (d) mouse-specific MHC Class I H-2KD or -Sca-1 in tumour cells. Mean±s.d. (n=4). (e) Immunofluorescence labelling of TRA-1-60, CD151 and CD166 in primary spheres and 4′-6-diamidino-2-phenylindole (DAPI) represents counterstain. Scale bar, 50 μm. (f) Percent combinatorial expression of the triple-markers in tumour cells. Mean±s.d. (n=8). (g) Marker-specific primary sphere-formation efficiency (determined as above in b). Mean±s.d. (n=5).
