Figure 4: Triple-marker expression in other prostate tumours and patient specimens.
From: Tumour-initiating stem-like cells in human prostate cancer exhibit increased NF-κB signalling
(a) Comparative immunohistochemical characterization of DU-145, PC3, VCaP and PC-82 tumours. Scale bar, 50 μm. (b) Percent marker-positive cells maintained after sequential in vivo passages of DU-145 tumours that were initially derived from the triple-marker-positive cells. Blue, passage1 and light blue, passage2. Mean±s.d. (n=4). (c) In vitro primary sphere-formation and (d) In vivo tumour initiation. Prospectively isolated 2,500 marker-positive DU-145 tumour cells were used. Tumour size was determined as tumour volume. Mean±s.d. (n=4). Unsorted control represents single cells of tumour. (e) Immunofluorescence labelling of markers in fresh frozen human patient primary prostate tumour specimens, with 4′-6-diamidino-2-phenylindole (DAPI) counterstain. Scale bar, 100 μm. (f) Percent marker-positive cells in human patients' prostate tumour specimens. Mean±s.d. (n=6). (g) Percent marker-positive cells in a variety of epithelial tumour specimens from clinical patients. Red, tumour cells and green, patient-matched normal tissue cells. Mean±s.d. (n=3).
