Table 1 Tumour initiation by sphere cells and purified tumour cells.
From: Tumour-initiating stem-like cells in human prostate cancer exhibit increased NF-κB signalling
Cell number | Cell phenotype | SC transplants | OT transplants | Tumour onset time (weeks) |
|---|---|---|---|---|
Human-versus mouse cells | ||||
1,000,000 | Total tumour cells | 2/2 | 2/2 | 4–6 |
200,000 | (MHC) HLA-class I ABC+ tumour cells | 2/2 | 2/2 | 4–6 |
200,000 | (MHC) HLA-class I ABC− tumour cells | 0/2 | 0/2 | NA |
200,000 | MHC class I H-2Kd+ tumour cells | 0/2 | 0/2 | NA |
Tumour versus sphere cells | ||||
5,000 | Total tumour cells | 0/9 | 0/2 | NA |
5,000 | Primary Sphere cells | 4/4 | 3/3 | 4–6 |
5,000 | Secondary sphere cells | 4/4 | 3/3 | 4–6 |
5,000 | Tertiary sphere cells | 4/4 | 2/2 | 4–6 |
Marker specificity | ||||
5,000 | EpCAM+ tumour cells | 5/5 | 3/3 | 4–6 |
5,000 | CD44+ tumour cells | 5/5 | 3/3 | 4–6 |
5,000 | α2-Integrin+ tumour cells | 5/5 | 3/3 | 4–6 |
5,000 | β4-Integrin+ tumour cells | 0/5 | 0/2 | NA |
5,000 | Sca1+ tumour cells | 0/4 | 0/4 | NA |
5,000 | Human PrEC (non-tumourigenic) cells | 0/3 | 0/2 | NA |
5,000 | Human RWPE1 (immortalized and untransformed) cells | 0/3 | 0/2 | NA |
5,000 | TRA-1-60+ tumour cells | 10/11 | 4/4 | 4–6 |
5,000 | PSMA+ tumour cells | 0/3 | 0/2 | NA |
5,000 | TRA-1-60− tumour cells | 0/3 | 0/4 | NA |
5,000 | PSMA+/TRA-1-60+ tumour cells | 3/3 | 2/2 | 4–6 |
5,000 | TRA-1-60+ primary sphere cells | 4/4 | 2/2 | 4–6 |
5,000 | TRA-1-60+ secondary sphere cells | 2/2 | 2/2 | 4–6 |
5,000 | TRA-1-60+ tertiary sphere cells | 2/2 | 2/2 | 4–6 |
Combinatorial marker expression | ||||
5,000 | CD 151+ tumour cells | 5/5 | 3/3 | 4–6 |
5,000 | CD 166+ tumour cells | 6/6 | 3/3 | 4–6 |
5,000 | Triple marker (TRA-1-60+CD151+CD166)+ tumour cells | 5/5 | 6/6 | 2–3 |
5,000 | TRA-1-60+/CD 151−/CD166− tumour cells | 0/3 | 0/2 | NA |
5,000 | TRA-1-60−/CD 151+/CD166− tumour cells | 0/3 | 0/2 | NA |
5,000 | TRA-1-60−/CD 151−/CD166+tumour cells | 0/3 | 0/2 | NA |
5,000 | TRA-1-60−/CD 151−/CD166−tumour cells | 0/3 | 0/2 | NA |
5,000 | Triple-marker-negative tumour cells | 0/3 | 0/3 | NA |
