Figure 1: Determination of the transcriptional architecture of the S. coelicolor genome. | Nature Communications

Figure 1: Determination of the transcriptional architecture of the S. coelicolor genome.

From: The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Figure 1

(a) Example of a dRNA-seq profile mapped onto the S. coelicolor genome. For TSS determination, total RNA samples from 44 growth conditions were pooled and two sequencing libraries were constructed, one from TEX-treated (TEX+) and the other from untreated total RNA (TEX−); TEX, terminator-5′-phosphate-dependent exonuclease. The criteria for assigning TSS are detailed in the Methods. (b) A total of 3,570 TSSs were identified and classified according to their positions relative to adjacent open reading frames (ORFs). TSSs located from 500 bp upstream to 150 bp downstream of the respective annotated start codon of each ORF were classified as the primary (P) or secondary (S). TSSs located within an annotated ORF or on the opposite strand were classified as internal (I) or antisense (A), respectively. TSSs not included in any of these categories were classified as intergenic (N). (c) Mapped TSSs in relation to those reported from previous studies. I, this study; II, ref. 16.; III, ref. 18. (d) TSSs associated with secondary metabolic gene clusters; prodiginine (left), bacteriocin (middle) and siderophore (right). (e) Proportion of each nucleotide at TSS (+1) and 2 nt upstream and downstream of the TSS.

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