Figure 4: Determination of the translatome of S. coelicolor.

(a) An example of visualization of transcription start sites (TSS), mRNA expression profiles (RNA) and ribosome-protected fragment profiles (RPF) at genomic region between 4,924,959 and 4,969,730. (b) RPF read data for the first and the second gene in the operon were compared at four growth phases: M, mid-exponential phase; T, transition phase; L, late exponential phase; S, stationary phase. (c) RPF data for ribosomal proteins RplJ and RplL (stoichiometry=1:4) and ATP synthase operon encoding AtpB, AtpE, AtpF, AtpH, AtpA, AtpG, AtpD and AtpC (stoichiometry=1:10:2:1:3:1:3:1) show proportional relationships with their subunit stoichiometry.