Figure 5: Translational buffering revealed by comparison of changes in mRNA and ribosome-protected fragment abundances. | Nature Communications

Figure 5: Translational buffering revealed by comparison of changes in mRNA and ribosome-protected fragment abundances.

From: The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Figure 5

(a) Distribution of mRNA fold-change and RPF fold-change of total genes, primary metabolic genes and secondary metabolic genes; ***P<0.001 (Wilcoxon signed-rank test); T, fold-change between mid-exponential and transition phases; L, fold-change between mid- and late exponential phases; S, fold-change between mid-exponential and stationary phases. (b) Negative correlation between changes in mRNA levels and translational efficiency (TE) becomes higher at later growth phases. Red dots indicate secondary metabolic genes. (c) TE change distributions of umRNAs and lmRNAs across growth phases. *P<0.05; ***P<0.001 (Wilcoxon rank-sum test). (d) G+C content of translation initiation regions (TIR: 20 nt sequence upstream of start codon); high, genes with high TE (upper 20%); total, all coding sequences; low, genes with low TE (lower 20%). (e) Correlation between free energy of TIR and TE. (f) Conserved ribosome-binding sequences for umRNAs were observed at 8–12 bp upstream region of start codon; lmRNAs, 5′-UTR length=0. TIR of genes with high TE (High) shows more conserved polypurine (G>A) motif than genes with low TE (Low).

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