Figure 1: IELs in 2D2 mice. | Nature Communications

Figure 1: IELs in 2D2 mice.

From: Gut environment-induced intraepithelial autoreactive CD4+ T cells suppress central nervous system autoimmunity via LAG-3

Figure 1

(a) Immunohistochemistry of the small intestines of WT and 2D2 mice. Cryosections were stained with anti-Vβ11 Ab (green) and nuclear DNA-staining DAPI (blue). Original magnification: × 600. Scale bars, 50 μm. Images shown are representative of two experiments. (b) Expression of Vα3.2+Vβ11+ TCR (2D2-TCR) in TCRβ+ IELs of WT or 2D2 mice was assessed by flow cytometry. 2D2-mouse IEL T cells with high expression of 2D2-TCR (2D2-IEL-THIGH) are indicated as THIGH, and those with low expression of 2D2-TCR (2D2-IEL-TLOW) are labelled as TLOW. (c) Expression of co-receptors (CD4 and CD8α) on 2D2-IEL T cells. (d) Expression of CD8β on CD8α+ T cells among 2D2-IEL-THIGH and -TLOW cells. (e) Expression of CD2 and CD5 on 2D2-IEL cells and 2D2-spleen CD4+ T cells (CD4+2D2-Sp-T). 2D2-IEL populations were analysed and grouped as CD4+2D2-IEL-THIGH, CD4−-2D2-IEL-THIGH and 2D2-IEL-TLOW cells. (f) Expression of CD103 and T-cell activation markers on 2D2-IEL cells and 2D2-spleen CD4+ T cells. (b–f) Data are representative of more than three experiments. DAPI, 4,6-diamidino-2-phenylindole.

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