Figure 9: The proliferation of CD4+2D2-IEL-THIGH cells in non-immunized mice. | Nature Communications

Figure 9: The proliferation of CD4+2D2-IEL-THIGH cells in non-immunized mice.

From: Gut environment-induced intraepithelial autoreactive CD4+ T cells suppress central nervous system autoimmunity via LAG-3

Figure 9

In total, 1.5 × 105 2D2-IEL-THIGH cells (CD45.2+) were labelled with CellTrace and transferred intravenously to CD45.1+WT mice. Five days later, cervical lymph node (LN) cells, inguinal LN cells, MLN cells, spleen cells and small intestinal IELs and LPL were collected. 7AAD−CD45.2+CD45.1−CD4+ cells were gated and CellTrace intensities were measured by FACS. Samples are pooled from two mice. Data represent three samples from two experiments. FACS, fluorescence-activated cell sorting.

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