Figure 3: mthfd1-1 mostly interferes with non-CG and H3K9 methylation. | Nature Communications

Figure 3: mthfd1-1 mostly interferes with non-CG and H3K9 methylation.

From: MTHFD1 controls DNA methylation in Arabidopsis

Figure 3

(a–c) Overlap between hypo-DMRs of different mutants in CG (a), CHG (b) and CHH (c) contexts. (d–f) CG (d), CHG (e) and CHH (f) methylation levels in DMR fractions corresponding to (a–c), respectively. w=wild-type, m=met1, t=mthfd1-1, c3=cmt3, c2=cmt2, d=drm1,2. Box plot (herein and after): horizontal line, median; edges of boxes, 25th (bottom) and 75th (top) percentiles; error bars, minimum and maximum points within 1.5 × interquartile range. (g) Heat map of DNA methylation levels in mthfd1-1 hypo-DMRs (rows) clustered by methylation levels. (h) Overlap of met1 or mthfd1-1 CG hypo-DMRs with PCGs or TEs. (i) Fluorescence micrographs of representative nuclei from WT and mthfd1-1. DNA was stained with DAPI and H3K9me2 was immunostained using Alexa Fluor 647 as secondary antibody. Scale bar, 5 μm. (j) Number of nuclei classified by DAPI staining and H3K9me2 immunofluorescence.

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