Figure 8: mthfd1-1 mutants are hypersensitive to exogenous 5-CHO-THF but tolerant to exogenous methionine.

(a) Root growth of seedlings on solid media containing mock, Met, 0.5 mM 5-CHO-THF, 0.5 mM 5-CH3-THF or 5 μM SMZ. Mean values±s.d. (n=3) are shown. (b) Average CG, CHG and CHH methylation levels at previously defined mthfd1-1 CG, CHG and CHH hypo-DMRs, respectively, in seedlings grown for 14 days on solid media containing mock, 0.1 mM Met, 0.5 mM 5-CHO-THF, 0.5 mM 5-CH3-THF or 5 μM SMZ. Two biological replicates are shown, except for SMZ. * indicates significant difference between mock and chemical treatment (P<0.05, Student’s t-test).