Figure 6: Characterization of klf2a−/− mutants.
From: klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis
(a) anti-fibronectin staining (green) is reduced in the AVC of trpp2−/− mutants (12/12 trpp2+/+, 9/19 trpp2−/−). (b,c) A range of valvular defects were observed in klf2a−/− mutant embryos at 96 hpf (n=25 klf2a+/+, n=48 klf2a−/−), similar to those observed in klf2aMO-injected embryos (e; n=20 control, n=17 klf2aMO). (d) Photoconversion of AVC EdCs at 48 hpf shows the organization of cells within the cardiac jelly to be affected by loss of klf2a (d,e; n=21 klf2+/+, n=32 klf2a−/−) at 72 hpf. Scale bars, 10 μm.
