Figure 1: MAIT cell activation during acute viral infections in vivo.
From: MAIT cells are activated during human viral infections
PBMC’s from healthy controls (HC, n=5–10), patients suffering from severe dengue (DHF, n=2–10), dengue (DF, n=4–10) or acute, hospitalized patients infected with influenza A virus (n=7–9) were analysed by flow cytometry by gating on live CD3+CD8+CD161++Vα7.2+ (MAIT) cells. (a,d,g) Representative flow cytometry plots. (b,e,h) Comparison between acute (day 0) and convalescent phase (day >10) of infection. (c,f,i) Comparison between DHF and DF patients. (j–m) Acute, hospitalized patients infected with influenza virus. (b,c,j,k) MAIT cell frequency as a proportion of the CD8+ T cell population. (e,f) Percentage of CD38 expression by MAIT cells. (h,i,l,m) Percentage of Granzyme B expression by MAIT cells. Statistical significance was determined with a Wilcoxon matched-paired test (b,e,h) or Mann–Whitney test (j,k). Bars represent means±s.e.m. ns>0.05, *P 0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001. conv, convalescent; Gr B, Granzyme B; HC, healthy control; ICU, intensive care unit.
