Figure 1: Increased ASC circulation in obese prostate cancer patients. | Nature Communications

Figure 1: Increased ASC circulation in obese prostate cancer patients.

From: CXCL1 mediates obesity-associated adipose stromal cell trafficking and function in the tumour microenvironment

Figure 1

(a) Flow cytometric enumeration of CD34brightCD45−CD31− ASCs, CD34brightCD45bright leukocytes, CD34brightCD31dimCD45dim CPCs (EPC/HPC) and CD34dimCD31brightCD45− endothelial cells in PBMCs of 12 healthy non-obese donors versus 24 non-obese and 21 obese prostate cancer patients. Graphs show mean±s.e.m.; *P<0.05 versus cancer-free non-obese donor; **P<0.05 versus non-obese cancer patient (Mann–Whitney U-test). (b) Representative bright-field micrographs of adherent cells recovered from equal numbers of PBMCs from representative non-obese and obese prostate cancer patients. Arrowheads: cells with monocyte morphology, arrows: cells with ASC morphology. (c) Three-lineage differentiation of obese patient PBMC-derived stromal cells expanded in culture (b). For >70% of culture area analysed, adipogenesis is evident from Oil red O staining of lipid droplets (red); osteogenesis is evident from Von Kossa staining for mineralization (black); and chondrogenesis is evident from toluidine blue staining type II collagen (purple). Arrows: differentiation area. Scale bar, 50 μm (b,c). Experiments (b,c) were performed for two obese patients with similar results.

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