Figure 1: Intrathymic T-cell development was severely blocked in Cxxc1-deficient mice. | Nature Communications

Figure 1: Intrathymic T-cell development was severely blocked in Cxxc1-deficient mice.

From: CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation

Figure 1

(a) Quantification of DN, DP, CD4+ and CD8+ SP thymocyte subpopulations. The statistical significance was calculated by unpaired t-test (two tailed). ***P<0.001. NS, not significant. Error bars indicate s.d. (b) Flow cytometry analysis of the thymocytes from indicated mice using anti-CD4 and anti-CD8 antibodies. (c) Lineage-negative cells were analysed for profile of DN1–DN4 subsets: DN1 (CD44+CD25−), DN2 (CD44+CD25+), DN3 (CD44−CD25+) and DN4 (CD44−CD25−). (d) Quantitation of the absolute cell numbers of CD4+ and CD8+ T-cell subsets, as the product of total splenocytes multiplied by the percentage of cells found in that population. (e,f) Total splenocytes were analysed for CD4, CD8, CD44 and CD62L expression by flow cytometry. The statistical significance was calculated by unpaired t-test (two tailed). ***P<0.001. Error bars indicate s.d. Data are from six-mice analysis (a–f).

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