Figure 5: Cxxc1 was crucial for TCR signalling in DP thymocytes.

(a) Flow cytometry analysis of CD69, CD5 and TCRβ in indicated DP thymocytes. Data are from three-mice analysis. (b) DP thymocytes sorted form indicated mice were cultured with medium alone (unstimulated; left), or with anti-TCRβ and anti-CD2 overnight and analysed immediately (middle), or washed and incubated for another 24 h in medium without antibodies (right). And analysis of live cells by flow cytometry. Data comes from three independently sorted DP cell sets (each from three mice). (c,d) Flow cytometry of thymocytes from control or Cxxc1-deficient mice bearing a transgene encoding the MHC class I-restricted OT-I TCR (c) or the MHC class II-restricted OT-II TCR (d). Left panel, staining of anti-CD4 and anti-CD8 on total thymocytes. Middle panel, staining with antibody to the OTI-specific variable region Vα5 (c) or the OTII-specific variable region Vβ5 (d). Right panel, quantitation of total thymocytes, CD8 SP thymocytes (c) or CD4 SP thymocytes (d). Data are from four-mice experiments. The statistical significance was determined by unpaired t-test (two tailed). ***P<0.001. Error bars indicate s.d.