Figure 3: Applications of AgDD.
From: A method to rapidly create protein aggregates in living cells
(a) HEK293 cells expressing AgDD and HEK293 cells expressing mCherry were mixed and co-cultured in media containing S1. S1 was withdrawn for the indicated times, and cell populations were quantified using flow cytometry. Replicates of n=3. Error bar is Standard Deviation (STD). (b) Time-lapse images of representative HEK293 cells stably expressing Nuclear AgDD. Top: cells cultured in media with S1 throughout the experiment; bottom: cells cultured in media with S1 withdrawn at 0 h. Scale bars, 5 μm. (c) Early time-lapse images of representative HEK293 cells stably expressing nuclear AgDD following S1 removal. Scale bar, 5 μm. (d–g) Images of representative nematodes expressing AgDD in intestinal cells. Top left: no S1; top right: 50 μM S1, bottom left: S1 removed for 2 h, bottom right: S1 removed for 6 h. Scale bar, 50 μm.
