Figure 1: ACF7 regulates epidermal migration in vivo.
From: In vivo epidermal migration requires focal adhesion targeting of ACF7
(a) Image of nude mouse grafted with organotypic skin culture (left panel). Arrows denote grafted skin. Intravital imaging shows efficient incorporation of grafted cells expressing luciferase (arrows) on engraftment (right panel). (b) Sections of nude skin with grafted H2B–RFP-expressing keratinocytes were stained with DAPI. Dotted lines denote dermal–epidermal boundaries. Arrow heads denote the boundary between grafted skin and host skin. Scale bar, 50 μm. Epi, epidermis; Der, dermis; HF, hair follicle. (c) Wound healing as monitored by histological staining of skin sections at the wound edges 6 days after injury. Halves of wound sections are shown. Dotted lines denote dermal–epidermal boundaries. Wounding will induce proliferation and migration of epidermal keratinocytes, which lead to formation of hyperproliferative epidermis (HE) at the wound edge. Note significantly longer HE in the WT grafted skin comparing with the KO counterpart. Es, eschar. Scale bar, 100 μm. (d) Quantification of the length of HE generated at times indicated after wounding (left panel). Error bars represent s.e.m. *P<0.05 (student t-test). The right panel shows the quantification of Ki67-positive cells present in wound HE. Error bars represent s.d. The difference is not statistically significant (P=0.58). (e) Intravital imaging of H2B-GFP expression skin cells with multiphoton microscopy. The dashed line denotes wound edge. The red lines in the right panel show migration trajectory of each cell. Scale bar, 50 μm. (f) Plotting of individual epidermal cell-movement trajectories in vivo. The arrowhead indicates the direction towards wound centre. (g) Box and whisker plots of cell velocities in vivo. The plot indicates the mean (solid diamond within the box), 25th percentile (bottom line of the box), median (middle line of the box), 75th percentile (top line of the box), 5th and 95th percentile (whiskers), 1st and 99th percentile (solid triangles) and minimum and maximum measurements (solid squares). *P<0.05 (student t-test). (h) Windrose plotting of cell-movement directions. The arrow head indicates the direction towards wound centre. (i) Quantification of cell-movement persistency in vivo. Error bar represents s.d. *P<0.05 (student t-test).
