Figure 2: Crystal structure of ACF7-NT.
From: In vivo epidermal migration requires focal adhesion targeting of ACF7
(a) Diagram of ACF7 domain structure. The following abbreviations are used: CH, calponin homology domain; SR1, spectrin repeat 1 (of Plakin domain); EF, EF hand motif; GAR-GSR, Gas2 related and GSR repeats for microtubule-binding domain. The second CH domain in the N terminus contains the tyrosine-phosphorylation site (phosphor-tyrosine in red). (b) Immunofluorescence for ACF7 (green), F-actin or focal adhesions/vinculin (red), and microtubules (blue) shows enriched ACF7 staining at the tips of actin fibres and focal adhesions. Boxed areas are magnified as insets. Scale bar, 20 μm. (c) ACF7-NT structure. Two tandem CH domains and the SR1 of Plakin domain are marked. (d) The three potential F-actin-binding sites (ABS1–3) were partially hidden in the closed conformation of tandem CH domains in ACF7’s ABD. ABS1, cyan; ABS2, green; and ABS3, orange.
