Figure 2: Chimaeric sgRNAs allow recruitment of fluorescent proteins and efficient labelling of major satellites. | Nature Communications

Figure 2: Chimaeric sgRNAs allow recruitment of fluorescent proteins and efficient labelling of major satellites.

From: CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

Figure 2

(a,b) Expression of either sgMajSat-MS2 or sgMajSat-PP7 sgRNAs in cells expressing dCas9, MCP-EGFP and PCP-mCherry leads to efficient labelling of pericentromeric regions in green or red, respectively (top panels). Fluorescent protein signals were validated using FISH with a γ-satellite probe (bottom panels). (c) The signal obtained with sgRNAs was compared with dCas9-EGFP fusion protein signal. Signal to background ratio was measured from raw images. The long dashed line across the entire plot represents the averaged intensity of background. The short dashed lines indicate the highest value of each peak, which is the highest intensity of each PCH punctum. Values next to vertical arrows represent the signal-to-background ratio.

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