Figure 4: Imaging of single loci on the mouse chromosome 12. | Nature Communications

Figure 4: Imaging of single loci on the mouse chromosome 12.

From: CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

Figure 4

(a) Scheme of sgRNAs designed to target Igh and Akap6 on mouse chromosome 12. A single sgRNA targets 87 locations on the Akap6 gene across 6.2 kb. Thirteen different sgRNAs were designed to target four different Igh switch regions at the telomeric end of mouse chromosome 12. The number of hits for each sgRNA is written below the bar representing its location (b) 3T3 fibroblasts expressing dCas9, MCP-EGFP and PCP-mCherry were co-infected with virus targeting the Akap6 gene (sgAkap6-PP7) and the 4 Igh switch regions (sgIgh-MS2). (c) Cytogenetic analysis of chromosome 12 and Igh on 3T3 fibroblasts using metaphase-FISH with a mouse chromosome 12 paint and 2 BAC probes surrounding Igh (Igh 5′ and Igh 3′).

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