Figure 1: ES9 inhibits CME in different systems. | Nature Communications

Figure 1: ES9 inhibits CME in different systems.

From: Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification

Figure 1

(a) Chemical structure of ES9. (b) Strongly reduced FM4-64 uptake (2 μM, 30 min) in Arabidopsis root cells in the presence of ES9 (10 μM) when compared with mock treatment (DMSO, Ø). (c) Super resolution immunofluorescence images of boutons of the neuromuscular junction (NMJ) in third instar Drosophila larvae positive for HA-tagged clathrin heavy chain (HA-CHC) comparing mock with 10 μM ES9 (30 min), in both non-stimulated (NS) or 90 mM KCl-stimulated NMJs to induce uptake. (d) Boxplot representation of transferrin uptake in HeLa cells after 20 min in the presence of 50 μM dynasore and 10 or 50 μM ES9. The signal intensity is plotted relative to mock (DMSO) and shows a concentration-dependent inhibition of uptake by ES9. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots. The box width is relative to sample size (n=35, 29, and 61 cells). Scale bars, 5 μm in b; 2 μm in c.

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