Figure 3: ES9 is a mitochondrial uncoupler. | Nature Communications

Figure 3: ES9 is a mitochondrial uncoupler.

From: Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification

Figure 3

(a) ATP measurements in the presence of 10 μM ES9, 1 μM carbonyl cyanide m-chlorophenyl hydrazine (CCCP), 50 μM tyrphostinA23 (TyrA23), 50 μM TyrA51, 20 μM antimycin A (AA), and 10 μM oligomycin (Oligo), showing various degrees of ATP depletion relative to mock treatment. Results were combined from three independent experiments. Error bars, s.e.m. (b) Confocal images of labelled mitochondria in Arabidopsis root cells (250 nM MitoTracker Red CM-H2XRos) in the presence of 10 μM ES9, 1 μM CCCP, 50 μM TyrA23, 50 μM TyrA51, 20 μM AA, and the mock (DMSO, Ø) for 30 min. The absence of mitochondrial staining implies that the electron transport is uncoupled or inhibited. (c) Tetramethylrhodamine ethyl ester (TMRE) labelling of D42mitoGFP positive mitochondria in Drosophila neuromuscular junctions compared to mock (1% DMSO (v/v) in HL-3 medium, 15 min) with 10 μM ES9 (30 min pre-treatment, 15 min treatment) and 10 μM CCCP (15 min). Reduced TMRE staining points to disruption of the mitochondrial function and reveal more depolarized mitochondria. Scale bars, 5 μm.

Back to article page