Figure 1: BRAT1 contributes to transcriptional anti-silencing. | Nature Communications

Figure 1: BRAT1 contributes to transcriptional anti-silencing.

From: The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing

Figure 1

(a) The RNA transcripts of AtGP1, AtMU1 and SDC were detected by quantitative RT–PCR in the WT (wild-type) col-0 and the two individual brat1 mutant alleles brat1-1 and brat1-2. The actin gene ACT7 was amplified as an internal control. Asterisks indicate statistical significance as determined by Student’s t-test (**P<0.01; *P<0.05). (b) The effect of brat1 on the expression of RD29A-LUC and 35S-NPTII transgenes was determined by luminescence imaging and kanamycin sensitivity, respectively. The RD29A-LUC and 35S-NPTII reporter genes were introduced into the brat1-1 mutant by crossing. (c) The RNA transcripts of RD29A-LUC and 35S-NPTII were detected by quantitative RT–PCR. Error bars represent s.d. of three biological replicates.

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